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viability marker fixable viability dye efluor 780  (Thermo Fisher)


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    Thermo Fisher viability marker fixable viability dye efluor 780
    Viability Marker Fixable Viability Dye Efluor 780, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/viability+marker+fixable+viability+dye+efluor+780/fixable+viability+dye/pm36827420-335-33-38
    Average 90 stars, based on 1 article reviews
    viability marker fixable viability dye efluor 780 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Bispecificity for myelin and neuronal self-antigens is a common feature of CD4 T cells in C57BL/6 mice.
    Article Snippet: On the fol- at U niversity of A uckland on M arch 3, 2015 http://w w w .jim m unol.org/ D ow nloaded from lowing day, cells were incubated for 4 h with GolgiPlug (containing brefeldin A; BD Biosciences). .. Cells were then stained with a viability marker (Fixable Viability Dye eFluor 780; eBioscience) and the following mix of Abs: PE-Cyanine 7-CD90.2 (clone 53-2.1; eBioscience), Pacific Blue– CD4 (clone RM4-5; BD Pharmingen), PerCP-Cy5.5-CD8b (clone YTS156.7.7; BioLegend, San Diego, CA), Alexa Fluor 700–CD44 (clone IM7; eBioscience), allophycocyanin-anti–IFN-g (clone XMG1.2; BD), and FITC-anti–IL-17 (clone TC11-18H10; BD Biosciences) Abs using a Cytofix/cytoperm PLUS kit (BD Biosciences). .. Stained cells were acquired on a BD Biosciences LSR II flow cytometer, and data were analyzed with BD Biosciences FACSDiva software version 6.2.

    Article Title: Attached stratified mucus separates bacteria from the epithelial cells in COPD lungs
    Article Snippet: .. Cell suspensions from the different fractions, CD45 + (leukocytes), Ly-6G + (neutrophils), and CD45 – Ly-6G – were washed in FACS buffer (HBSS without calcium, magnesium, and phenol red [HBSSwo], 2% v/v FCS, 5 mM EDTA) and stained with the viability marker Fixable Viability Dye eFluor 780 (Thermo Fisher Scientific) for 30 minutes, on ice and in the dark. .. After the viability staining, cells were washed in FACS buffer and labeled with anti–EpCAM FITC (G8.8), anti–CD45 PE (30-F11) and anti–Ly-6G-PerCP eFluor710 (1A8-Ly6g) (all from Thermo Fisher Scientific).

    Article Title: IL-15 Promotes Polyfunctional NK Cell Responses to Influenza by Boosting IL-12 Production
    Article Snippet: .. Cells were stained in 96-well round-bottom plates for surface markers, including viability marker (Fixable Viability Dye eFluor 780; eBioscience) in FACS buffer (PBS containing 0.5% FCS, 0.05% sodium azide, and 2 mM EDTA) for 30 min after blocking Fc receptors for 5 min with Fc Receptor Blocking Reagent (Miltenyi Biotec). .. Cells were then washed in FACS buffer and fixed and permeabilized using a BD Cytofix/Cytoperm Kit, according to the manufacturer’s instructions.

    Article Title: Attached stratified mucus separates bacteria from the epithelial cells in COPD lungs.
    Article Snippet: .. Cell suspensions from the different fractions, CD45+ (leukocytes), Ly-6G+ (neutrophils), and CD45–Ly-6G– were washed in FACS buffer (HBSS without calcium, magnesium, and phenol red [HBSSwo], 2% v/v FCS, 5 mM EDTA) and stained with the viability marker Fixable Viability Dye eFluor 780 (Thermo Fisher Scientific) for 30 minutes, on ice and in the dark. .. After the viability staining, cells were washed in FACS buffer and labeled with anti–EpCAM FITC (G8.8), anti–CD45 PE (30-F11) and anti–Ly-6G-PerCP eFluor710 (1A8-Ly6g) (all from Thermo Fisher Scientific).

    Marker:

    Article Title: Bispecificity for myelin and neuronal self-antigens is a common feature of CD4 T cells in C57BL/6 mice.
    Article Snippet: On the fol- at U niversity of A uckland on M arch 3, 2015 http://w w w .jim m unol.org/ D ow nloaded from lowing day, cells were incubated for 4 h with GolgiPlug (containing brefeldin A; BD Biosciences). .. Cells were then stained with a viability marker (Fixable Viability Dye eFluor 780; eBioscience) and the following mix of Abs: PE-Cyanine 7-CD90.2 (clone 53-2.1; eBioscience), Pacific Blue– CD4 (clone RM4-5; BD Pharmingen), PerCP-Cy5.5-CD8b (clone YTS156.7.7; BioLegend, San Diego, CA), Alexa Fluor 700–CD44 (clone IM7; eBioscience), allophycocyanin-anti–IFN-g (clone XMG1.2; BD), and FITC-anti–IL-17 (clone TC11-18H10; BD Biosciences) Abs using a Cytofix/cytoperm PLUS kit (BD Biosciences). .. Stained cells were acquired on a BD Biosciences LSR II flow cytometer, and data were analyzed with BD Biosciences FACSDiva software version 6.2.

    Article Title: Attached stratified mucus separates bacteria from the epithelial cells in COPD lungs
    Article Snippet: .. Cell suspensions from the different fractions, CD45 + (leukocytes), Ly-6G + (neutrophils), and CD45 – Ly-6G – were washed in FACS buffer (HBSS without calcium, magnesium, and phenol red [HBSSwo], 2% v/v FCS, 5 mM EDTA) and stained with the viability marker Fixable Viability Dye eFluor 780 (Thermo Fisher Scientific) for 30 minutes, on ice and in the dark. .. After the viability staining, cells were washed in FACS buffer and labeled with anti–EpCAM FITC (G8.8), anti–CD45 PE (30-F11) and anti–Ly-6G-PerCP eFluor710 (1A8-Ly6g) (all from Thermo Fisher Scientific).

    Article Title: Dampening type 2 properties of group 2 innate lymphoid cells by a gammaherpesvirus infection reprograms alveolar macrophages.
    Article Snippet: Immunological dysregulation in asthma is associated with changes in exposure to microorganisms early in life.. Gammaherpesviruses (γHVs), such as Epstein-Barr virus, are widespread human viruses that establish lifelong infection and profoundly shape host immunity.. Using murid herpesvirus 4 (MuHV-4), a mouse γHV, we show that after infection, lung-resident and recruited group 2 innate lymphoid cells (ILC2s) exhibit a reduced ability to expand and produce type 2 cytokines in response to house dust mites, thereby contributing to protection against asthma.

    Article Title: IL-15 Promotes Polyfunctional NK Cell Responses to Influenza by Boosting IL-12 Production
    Article Snippet: .. Cells were stained in 96-well round-bottom plates for surface markers, including viability marker (Fixable Viability Dye eFluor 780; eBioscience) in FACS buffer (PBS containing 0.5% FCS, 0.05% sodium azide, and 2 mM EDTA) for 30 min after blocking Fc receptors for 5 min with Fc Receptor Blocking Reagent (Miltenyi Biotec). .. Cells were then washed in FACS buffer and fixed and permeabilized using a BD Cytofix/Cytoperm Kit, according to the manufacturer’s instructions.

    Article Title: Attached stratified mucus separates bacteria from the epithelial cells in COPD lungs.
    Article Snippet: .. Cell suspensions from the different fractions, CD45+ (leukocytes), Ly-6G+ (neutrophils), and CD45–Ly-6G– were washed in FACS buffer (HBSS without calcium, magnesium, and phenol red [HBSSwo], 2% v/v FCS, 5 mM EDTA) and stained with the viability marker Fixable Viability Dye eFluor 780 (Thermo Fisher Scientific) for 30 minutes, on ice and in the dark. .. After the viability staining, cells were washed in FACS buffer and labeled with anti–EpCAM FITC (G8.8), anti–CD45 PE (30-F11) and anti–Ly-6G-PerCP eFluor710 (1A8-Ly6g) (all from Thermo Fisher Scientific).

    FACS:

    Article Title: Attached stratified mucus separates bacteria from the epithelial cells in COPD lungs
    Article Snippet: .. Cell suspensions from the different fractions, CD45 + (leukocytes), Ly-6G + (neutrophils), and CD45 – Ly-6G – were washed in FACS buffer (HBSS without calcium, magnesium, and phenol red [HBSSwo], 2% v/v FCS, 5 mM EDTA) and stained with the viability marker Fixable Viability Dye eFluor 780 (Thermo Fisher Scientific) for 30 minutes, on ice and in the dark. .. After the viability staining, cells were washed in FACS buffer and labeled with anti–EpCAM FITC (G8.8), anti–CD45 PE (30-F11) and anti–Ly-6G-PerCP eFluor710 (1A8-Ly6g) (all from Thermo Fisher Scientific).

    Article Title: IL-15 Promotes Polyfunctional NK Cell Responses to Influenza by Boosting IL-12 Production
    Article Snippet: .. Cells were stained in 96-well round-bottom plates for surface markers, including viability marker (Fixable Viability Dye eFluor 780; eBioscience) in FACS buffer (PBS containing 0.5% FCS, 0.05% sodium azide, and 2 mM EDTA) for 30 min after blocking Fc receptors for 5 min with Fc Receptor Blocking Reagent (Miltenyi Biotec). .. Cells were then washed in FACS buffer and fixed and permeabilized using a BD Cytofix/Cytoperm Kit, according to the manufacturer’s instructions.

    Article Title: Attached stratified mucus separates bacteria from the epithelial cells in COPD lungs.
    Article Snippet: .. Cell suspensions from the different fractions, CD45+ (leukocytes), Ly-6G+ (neutrophils), and CD45–Ly-6G– were washed in FACS buffer (HBSS without calcium, magnesium, and phenol red [HBSSwo], 2% v/v FCS, 5 mM EDTA) and stained with the viability marker Fixable Viability Dye eFluor 780 (Thermo Fisher Scientific) for 30 minutes, on ice and in the dark. .. After the viability staining, cells were washed in FACS buffer and labeled with anti–EpCAM FITC (G8.8), anti–CD45 PE (30-F11) and anti–Ly-6G-PerCP eFluor710 (1A8-Ly6g) (all from Thermo Fisher Scientific).

    Selection:

    Article Title: Dampening type 2 properties of group 2 innate lymphoid cells by a gammaherpesvirus infection reprograms alveolar macrophages.
    Article Snippet: Immunological dysregulation in asthma is associated with changes in exposure to microorganisms early in life.. Gammaherpesviruses (γHVs), such as Epstein-Barr virus, are widespread human viruses that establish lifelong infection and profoundly shape host immunity.. Using murid herpesvirus 4 (MuHV-4), a mouse γHV, we show that after infection, lung-resident and recruited group 2 innate lymphoid cells (ILC2s) exhibit a reduced ability to expand and produce type 2 cytokines in response to house dust mites, thereby contributing to protection against asthma.

    Blocking Assay:

    Article Title: IL-15 Promotes Polyfunctional NK Cell Responses to Influenza by Boosting IL-12 Production
    Article Snippet: .. Cells were stained in 96-well round-bottom plates for surface markers, including viability marker (Fixable Viability Dye eFluor 780; eBioscience) in FACS buffer (PBS containing 0.5% FCS, 0.05% sodium azide, and 2 mM EDTA) for 30 min after blocking Fc receptors for 5 min with Fc Receptor Blocking Reagent (Miltenyi Biotec). .. Cells were then washed in FACS buffer and fixed and permeabilized using a BD Cytofix/Cytoperm Kit, according to the manufacturer’s instructions.



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